human umbilical artery endothelial cells (huaec) Search Results


human umbilical artery endothelial cells (huaec), cell pellet in rnalater  (PromoCell)
N/A
Cell pellet of HUAEC from the arteries of the umbilical cord in RNAlater® for subsequent RNA, DNA or protein analysis. Cell pellet consisting of 1 million cells dissolved in 200 µl RNAlater® for subsequent RNA,
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umbilical artery huaec  (Cell Applications Inc)
94
Cell Applications Inc umbilical artery huaec
Umbilical Artery Huaec, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+umbilical+artery+endothelial+cells+(huaec)/Human+Umbilical+Artery+Endothelial+Cells%3A+HUAEC/pm36383185-61-19-25
Average 94 stars, based on 1 article reviews
umbilical artery huaec - by Bioz Stars, 2026-09
94/100 stars
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human umbilical artery endothelial cells (huaec)  (ScienCell)
90
ScienCell human umbilical artery endothelial cells (huaec)
<t>Endothelial</t> ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control
Human Umbilical Artery Endothelial Cells (Huaec), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+umbilical+artery+endothelial+cells+(huaec)/human+umbilical+artery+endothelial+cells++huaec+/pmc10717156-42-0-8
Average 90 stars, based on 1 article reviews
human umbilical artery endothelial cells (huaec) - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Endothelial ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Endothelial ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Expressing, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Western Blot, Over Expression, Control

Downregulation of ABCG1 promoted endothelial apoptosis. Annexin V-FITC/PI double-staining assay was used to quantify apoptosis in HUAEC by means of flow cytometry. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+). a Apoptosis in endothelial control, b increased apoptosis in ABCG1-deficient EC, c apoptosis in ABCG1-deficient EC transfected with ABCG1 plasmid, d apoptosis in endothelial control transfected with ABCG1 plasmid

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Downregulation of ABCG1 promoted endothelial apoptosis. Annexin V-FITC/PI double-staining assay was used to quantify apoptosis in HUAEC by means of flow cytometry. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+). a Apoptosis in endothelial control, b increased apoptosis in ABCG1-deficient EC, c apoptosis in ABCG1-deficient EC transfected with ABCG1 plasmid, d apoptosis in endothelial control transfected with ABCG1 plasmid

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Double Staining, Flow Cytometry, Control, Transfection, Plasmid Preparation

Blocking ER stress reduced endothelial apoptosis in ABCG1-deficient EC. Compared with control cells (a), endothelial apoptosis was significantly induced in ABCG1-deficient EC (b). c Endothelial apoptosis was partly reversed in ABCG1-deficient EC treated with PBA. d Endothelial apoptosis was moderately but not statistically decreased in control cells treated with PBA. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+) as described in “Methods”

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Blocking ER stress reduced endothelial apoptosis in ABCG1-deficient EC. Compared with control cells (a), endothelial apoptosis was significantly induced in ABCG1-deficient EC (b). c Endothelial apoptosis was partly reversed in ABCG1-deficient EC treated with PBA. d Endothelial apoptosis was moderately but not statistically decreased in control cells treated with PBA. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+) as described in “Methods”

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Blocking Assay, Control

Cholesterol repletion and depletion were correlated with endothelial apoptosis and activation of ER stress. a Cyclodextrin (CD)-cholesterol loading of control cells increased FC and CE content. CD-cholesterol loading of ABCG1-deficient EC statistically increased CE content but not FC content. Cholesterol depletion with CD lowed FC and CE content in both endothelial control and ABCG1-deficient EC. b Cholesterol repletion induced endothelial apoptosis in endothelial controls. Cholesterol depletion significantly reversed endothelial apoptosis in ABCG1-deficient EC. Apoptosis was suppressed in ABCG1-deficient EC treated with U18666A, a cholesterol transport to ER inhibitor. c, d Cholesterol repletion increased the expression of GRP78 and CHOP, and cholesterol depletion reduced the expression of GRP78 and CHOP in ABCG1-deficient EC and control cells. e, f Addition of U18666A to ABCG1-deficient EC suppressed expression of GRP78 and CHOP. Data are mean ± SD (n = 3). *P = 0.05, **P < 0.001 vs. EC controls, # P < 0.001 vs. ABCG1-deficient EC. Representative images are shown for three independent experiments

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Cholesterol repletion and depletion were correlated with endothelial apoptosis and activation of ER stress. a Cyclodextrin (CD)-cholesterol loading of control cells increased FC and CE content. CD-cholesterol loading of ABCG1-deficient EC statistically increased CE content but not FC content. Cholesterol depletion with CD lowed FC and CE content in both endothelial control and ABCG1-deficient EC. b Cholesterol repletion induced endothelial apoptosis in endothelial controls. Cholesterol depletion significantly reversed endothelial apoptosis in ABCG1-deficient EC. Apoptosis was suppressed in ABCG1-deficient EC treated with U18666A, a cholesterol transport to ER inhibitor. c, d Cholesterol repletion increased the expression of GRP78 and CHOP, and cholesterol depletion reduced the expression of GRP78 and CHOP in ABCG1-deficient EC and control cells. e, f Addition of U18666A to ABCG1-deficient EC suppressed expression of GRP78 and CHOP. Data are mean ± SD (n = 3). *P = 0.05, **P < 0.001 vs. EC controls, # P < 0.001 vs. ABCG1-deficient EC. Representative images are shown for three independent experiments

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Activation Assay, Control, Expressing